Identifying a Detoxifying Uridine Diphosphate Glucosyltransferase (UGT), MdUGT83K2, Which Can Glycosylate the Aryloxyphenoxypropionate Herbicide

Glycosylation is a common modification reaction in plants. The products obtained upon glycosylation have different biological functions, making an important mechanism affecting and regulating the balance of plant growth metabolism. In this study, we first speculated that Group I apple glycosyltransferase family may predicted function like UGT83A1, according to gene chip data published online. Subsequently, by real-time PCR (polymerase chain reaction), analyzed whether expression nine genes was induced our previously reported ACCase (Acetyl-CoA carboxylase) inhibition-based herbicide QPP ((R)-ethyl·2-(4-((6-fluoro-3-methyl-4-oxo-3,4-dihydroquinazolin-2-yl)oxy) phenoxy) propanoate). It found MdUGT83K2 significantly increased QPP. order determine can glycosylate QPP, confirmed enzymatic vitro presence glycosides transgenic seedlings HPLC (High Performance Liquid Chromatography), transfer glucose vivo, which glycosylated. work, identified novel glycosyltransferase, MdUGT83K2, functions ACCase-inhibiting be involved detoxification. Key Contribution: A identified, detoxification herbicide.